Proteomics

We are equipped for proteomics research including Cell Fractionation on one floor and tabletop Ultracentrifuges (Beckman Coulter) , Fast Protein Liquid Chromatography (FPLC), Two-Dimensional Protein Electrophoresis (Protean II, Biorad), and Gel Documentation Systems (one Bio-Rad and one Fuji Medical). Protein quantification can be done using UV-Vis spectrophotometry ( DU650, Beckman Coulter) or multimode Plate Spectrophotometers in both 96-well and 384-well micro well plate formats. Protein gels can also be visualized, digitized, and documented on Multi-Mode Imager Typhoon 9410.

List of equipment for proteomics research in CCRTD:

Isoelectrofocusing unit with high-voltage power supply (Bio-Rad)
Fast Protein Liquid Chromatography System (FPLC)
High-speed tabletop ultra-centrifuge (Beckman Coulter)
NMR facility (supported by Chemistry Department)
Plate spectrophotometer with UV-Vis, Fluorescence, TOF, and luminescence detection modes in 96 and 384-well formats (BioTek)
Two-dimensional protein separation setup including accessories (Protean II, Bio-Rad)