Molecular Biology

CCRTD Core Facilities house major equipment for DNA and RNA-based applications. We offer polymerase chain reaction ( PCR ) instruments including Real-Time PCR machine (I-Cycle, Bio-Rad), Liquid Scintillation Counter (Beckman Coulter), Lyophilizer and Freeze-Dryer for biological samples, Cell Porator, high-speed and ultra-high-speed Centrifuges, ultra-sensitive Balances, and other supporting equipment. DNA and RNA quantification can be carried out on Spectrophotometers, both single-cuvette (DU650, Beckman Coulter) and 96-well plate based. DNA and RNA can be visualized, digitized, and quantified using Gel Documentation system (Bio-Rad) or Multi-Mode Imager Typhoon 9410, in fluorescent, luminescent, UV, or visible wavelengths; time-resolved fluorescence option is available on BioTek Plate Imager. For X-Ray imaging, we maintain an Automated X-Ray processor and Dark Room; radioisotope imaging is also supported on Typhoon 9410.

Here is the list of all major equipment and options for the support of molecular biology research in CCRTD in alphabetical order:

Autoclave (Tabletop)
Balance
Biological Imaging stations (Bio-Rad and Fuji Medical)
Cell electroporator
Centrifuges (two Beckman Coulter Avanti JE, one Eppendorf tabletop and one Beckman Coulter ultra)
Darkroom/Automated X-ray processor room
Deionized water source
Freeze Dryer
Freezer storage space, -20oC and -80oC
Micro plate spectrophotometers
Miscellaneous equipment – stirrers, hot plates, etc.
PCR instruments
Real-Time PCR machine
Refrigerator storage space
Scintillation Counter (liquid)
Sonicator
Speed-Vac
Spectrophotometer
Temperature controlled incubator
Temperature controlled incubator shaker
Vacuum Oven