Prostate cancer (PCa) is one of the common malignancies and the second leading cause of tumor-related mortality in American male. The treatment with bicalutamide (casodex), the most widely used androgen receptor (AR) antagonist, is a standard systemic PCa treatment. Unfortunately, after a certain time of bicalutamide treatment, most PCa patients usually evolve to an androgen independent or hormone refractory state and current antiandrogens become ineffective. The precise mechanisms of how AR antagonist function is abrogated in progressive PCa after androgen deprivation are the central importance for the development of new therapies and represent a major clinical challenge in PCa treatment.
Protein and its spatiotemporal distribution change play a central role in all of biological processes including PCa cell transition from androgen dependence into androgen independence. Recently, large-scale protein identifications from highly complex protein mixtures have been achieved with proteomic strategies. Based on the improvement of mass spectrometry (MS) and sample preparation such as peptide fractionation with strong cation exchange (SCX) column in high pressure liquid chromatography (HPLC), now, MS-based proteomics has made it possible to directly identify thousands of proteins expressed in various cell lines. Moreover, isobaric mass tagging techniques such as tandem mass tags (TMT) and isobaric tags for relative and absolute quantitation (iTRAQ) have been well established as effective approaches for analysis of protein expression with accurate and precise relative quantitation of peptides in multiple samples simultaneously. The advantages of high-throughput and high-sensitivity of large-scale proteome analysis facilitate understanding of various mechanisms of cancers including PCa and development of new diagnostic/prognostic biomarkers and therapeutic targets.
Jin Zou and Naoki Sugimoto, A role of the Trp-His Interaction in the Conformational Switch between Alpha-helix and Beta-sheet in Short Alanine-based Peptides, J. Chem. Soc., Perkin Trans. 2, 2000, (10), 2135-2140.
Jin Zou and Naoki Sugimoto, Complexation of Peptide with Cu2+ Responsible to Inducing and Enhancing the Formation of Alpha-helix Conformation, Biometals, 2000, 13(4), 349-359.
Jin Zou and Naoki Sugimoto, Effect of Extending the CD Reference Spectra to 168 nm on the Prediction of Protein Secondary Structures, Journal of Biochemistry, Molecular Biology and Biophysics (JBMBB), 2001, 5(1), 15-24.
Jin Zou, Katsushi Kajita, and Naoki Sugimoto, Cu2+ Inhibits the Aggregation of Amyloid Beta-peptide (1-42) in vitro, Angew. Chem. Int. Ed. Engl., 2001, 40(12), 2274-2277.
Jin Zou, Yiming Ye, Kristy Welshhans, Monica Lurtz, April L. Ellis, Charles Louis, Vincent Rehder, Jenny J. Yang, Expression and Optical Properties of Green Fluorescent Protein Expressed in Different Cellular Environments, Journal of Biotechnology, 2005, 119(4) 368-378.
Jin Zou, Aldebaran M. Hofer, Monica M. Lurtz, Giovanni Gadda, April L. Ellis, Ning Chen, Yun Huang, Angela Holder, Yiming Ye, Charles F. Louis, Kristy Welshhans, Vincent Rehder, and Jenny J. Yang, Developing Sensors for Real Time Measurement of High Ca2+ Concentrations, Biochemistry, 2007, 46(43), 12275-12288.
Angela N. Holder, April L. Ellis, Jin Zou, Ning Chen, and Jenny J. Yang, Facilitating Chromophore Formation of Engineered Ca2+ Binding Green Fluorescent Proteins, Archives of Biochemistry and Biophysics, 2009, 486(1), 27-34.
Ning Chen, Jin Zou, Siming Wang, Yiming Ye, Yun Huang, Giovanni Gadda, and Jenny J. Yang, Designing Protease Sensors for Real-time Imaging of Trypsin Activation in Pancreatic Cancer Cells, Biochemistry, 2009, 48(15), 3519-3526.
Jie Jiang, Yubin Zhou, Jin Zou, Yanyi Chen, Priya Pate, Jenny J. Yang and Edward M. Balog, Site-specific Modification of Calmodulin Ca2+ Affinity Tunes the Skeletal Muscle Ryanodine Receptor Activation Profile, Biochem. J. 2010, 432(1), 89-99.
Mark A. Rider, Jin Zou, Dana Vanlandingham, John Nuckois, Steve Higgs, Qiang Zhang, Michelle Lacey, Joohyun Kim, Guangdi Wang, and Young S. Hong, Quantitative Proteomic Analysis of O’nyong-nyong Virus Infection in the Anopheles Gambiae Midgut, submitted.